Generator
Part:BBa_K511821:Design
Designed by: Grant Robinson Group: iGEM11_MIT (2011-09-28)
Inducible Fluorescence/Repression Generator (UAS-Gal4-amCyan-miRFF4) MammoBlock Device
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 126
Illegal EcoRI site found at 571
Illegal PstI site found at 1123 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 126
Illegal EcoRI site found at 571
Illegal NheI site found at 154
Illegal PstI site found at 1123 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 126
Illegal EcoRI site found at 571
Illegal XhoI site found at 83
Illegal XhoI site found at 468 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 126
Illegal EcoRI site found at 571
Illegal PstI site found at 1123 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 126
Illegal EcoRI site found at 571
Illegal PstI site found at 1123 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.
Source
Consult constituent components.