Generator

Part:BBa_K511821:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)


Inducible Fluorescence/Repression Generator (UAS-Gal4-amCyan-miRFF4) MammoBlock Device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 126
    Illegal EcoRI site found at 571
    Illegal PstI site found at 1123
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 126
    Illegal EcoRI site found at 571
    Illegal NheI site found at 154
    Illegal PstI site found at 1123
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 126
    Illegal EcoRI site found at 571
    Illegal XhoI site found at 83
    Illegal XhoI site found at 468
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 126
    Illegal EcoRI site found at 571
    Illegal PstI site found at 1123
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 126
    Illegal EcoRI site found at 571
    Illegal PstI site found at 1123
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.


Source

Consult constituent components.

References